pluripotent stem cells human endothelial cells  (ATCC)

Journal: PLoS ONE

Article Title: Diabetes Mellitus Aggravates Hemorrhagic Transformation after Ischemic Stroke via Mitochondrial Defects Leading to Endothelial Apoptosis

doi: 10.1371/journal.pone.0103818

Figure Lengend Snippet: A: Experimental protocol in vitro . B: Nuclear staining for Hoechst 33342. The number of cells exhibiting nuclear stain was counted (n = 10). The scale bar indicates 50 µm. C: Number of TUNEL-positive cells (n = 4). The scale bar indicates 100 µm. D: Caspase-3/7 activities (n = 10). E: Immunoblotting for Active Caspase-3. F: Immunoblotting for Caspase-7. G: Release of apoptosis inducing factor (AIF) and cytochrome c (Cyto c ) into cytosol, and transit into the nucleus (n = 3). All data are expressed as mean ± SEM (shown as ratio to 5.5 mM). *P<0.05, **P<0.01 vs. 5.5 mM (Dunnet's test). HBMVECs, human brain microvascular endothelial cells.

Article Snippet: We also examined the effects of high-glucose exposure for 6 days on cell death, mitochondrial functions and morphology in human brain microvascular endothelial cells (HBMVECs) or human endothelial cells derived from induced pluripotent stem cells (iCell endothelial cells).

Techniques: In Vitro, Staining, TUNEL Assay, Western Blot

Journal: PLoS ONE

Article Title: Diabetes Mellitus Aggravates Hemorrhagic Transformation after Ischemic Stroke via Mitochondrial Defects Leading to Endothelial Apoptosis

doi: 10.1371/journal.pone.0103818

Figure Lengend Snippet: A: Cell proliferation (n = 7). B: ATP contents (n = 10). C: Mitochondrial membrane potential, determined by tetramethyl rhodamine methyl ester (TMRM) intensity (n = 10). The scale bar indicates 100 µm. D: reactive oxygen species (ROS) levels (n = 10). E: Binding of 4-hydroxy-2-nonenal (4-HNE), an indicator of lipoperoxidation by ROS. F: Enzymatic activities of MMP-2 and MMP-9 by gelatin zymography (n = 3). All data are expressed as mean ± SEM (shown as ratio to 5.5 mM). *P<0.05, **P<0.01 vs. 5.5 mM (Dunnet's test). HBMVECs, human brain microvascular endothelial cells.

Article Snippet: We also examined the effects of high-glucose exposure for 6 days on cell death, mitochondrial functions and morphology in human brain microvascular endothelial cells (HBMVECs) or human endothelial cells derived from induced pluripotent stem cells (iCell endothelial cells).

Techniques: Membrane, Binding Assay, Zymography

Journal: PLoS ONE

Article Title: Diabetes Mellitus Aggravates Hemorrhagic Transformation after Ischemic Stroke via Mitochondrial Defects Leading to Endothelial Apoptosis

doi: 10.1371/journal.pone.0103818

Figure Lengend Snippet: A: Characterization of iCell endothelial cells by immunostaining of CD31, a marker of endothelial cells. The scale bar indicates 20 µm. B: Temporal changes of cell proliferation, assessed at 1, 3, and 6 days after the onset of high-glucose exposure (n = 9 or 10). C: Nuclear staining for Hoechst 33342. The number of cells exhibiting nuclear stain was counted (n = 10). The scale bar indicates 50 µm. D: Number of TUNEL-positive cells (n = 4). The scale bar indicates 100 µm. All data are expressed as mean ± SEM (shown as percentage of 5.5 mM). *P<0.05, **P<0.01 vs. 5.5 mM (Tukey's test or Dunnet's test).

Article Snippet: We also examined the effects of high-glucose exposure for 6 days on cell death, mitochondrial functions and morphology in human brain microvascular endothelial cells (HBMVECs) or human endothelial cells derived from induced pluripotent stem cells (iCell endothelial cells).

Techniques: Immunostaining, Marker, Staining, TUNEL Assay